Conference publications

Physico-Mathematical Modelling of Target-Probe Binding on Microchips at Biological and Medical Investigations

Golovkin M. V., Matveeva O. V., Nechipurenko Yu. D.

"Математика. Компьютер. Образование". Cб. трудов XVI международной конференции. Под общей редакцией Г.Ю. Ризниченко Ижевск: Научно-издательский центр "Регулярная и хаотическая динамика", 2009. Vol. 2. Pp. 158-168. (accepted)

The process of microarray oligo-target hybridization was modeled using equilibrium adsorption. The relationship between hybridization signal intensity and Gibbs free energy change for oligo-target duplex formation was our function of interest. The equilibrium adsorption model predicts S-shaped functional relationship between these two variables. We called the respective curve Energetic Hybridization Isotherm (EHI). It was demonstrated that change of target/probe ratio could result in shifts of the inflection point of S-shaped EHI curve along both axes. This study provides insights into EHI curves’ behavior observed in microarray experiments.

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