Physico-Mathematical Modelling of Target-Probe Binding on Microchips at Biological and Medical Investigations
"Математика. Компьютер. Образование". Cб. трудов XVI международной конференции. Под общей редакцией Г.Ю. Ризниченко Ижевск: Научно-издательский центр "Регулярная и хаотическая динамика", 2009. Vol. 2. Pp. 158-168. (accepted)
The process of microarray oligo-target hybridization was modeled using equilibrium adsorption. The relationship between hybridization signal intensity and Gibbs free energy change for oligo-target duplex formation was our function of interest. The equilibrium adsorption model predicts S-shaped functional relationship between these two variables. We called the respective curve Energetic Hybridization Isotherm (EHI). It was demonstrated that change of target/probe ratio could result in shifts of the inflection point of S-shaped EHI curve along both axes. This study provides insights into EHI curves’ behavior observed in microarray experiments.