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Dynamic morphometry of planarian regeneration: growth and morphogenesis criteria

Tiras Kh.P., Deev AA1, Mestetsky LM2, Vorobyova UM, Klimenko S.V.

Pushchino State Institute of Natural Sciences, Russia, 142290, Pushchino, Science Prospect, 3, (4967) 731857, tiras1950@yandex.ru,

1Institute of Theoretical and Experimental Biophysics RAS, Russia, 142290, Pushchino, Institutskaya ul., 3, (495) 632-78-69, deev@iteb.ru

2Moscow State University, Faculty of Computational Mathematics and Cybernetics, Russia, 119991, Moscow, GSP-1, Leninskie Gory, 1, p.52, (495) 939-30-10, mestlm@mail.ru

Flatworms - planaria and their post-traumatic regeneration - a classic example of complete morphogenesis, and, at the same time, the optimal 2D object of computer biology. The planar structure of the planar body allows one to construct an accurate picture of the regeneration process on the basis of planimetric 2D planar images. Planarians do not have a rigid outer or inner skeleton, so to unify the registration of planaria in the norm and regenerate it is carried out along the course of its rectilinear uniform motion of the animal along the surface of the petri dish in the field of view of the microscope. A unique feature of planarians is the ability to regenerate their central nervous system - the head ganglion. Full regeneration involves two processes: growth and morphogenesis. When regenerating the head of the body, the regenerative bud - blastema - is formed and grows. Simultaneously, the morphogenesis of the blastema occurs - a change in its shape from a semicircular to a triangular one - which is characteristic of an intact animal.


For the analysis of planar images: , Girardia tigrina . in the norm and during regeneration of the head end of the body were used original software - Plana 5.0 and Wormeter 1.0, which allow to characterize the growth processes and morphogenesis of the growing blastema.
The size of the blastema was determined with the help of the interactive program Plana, where the operator determines the boundary of the growth. The size of the blastema directly depends on the number of cells that form it, so that the area of the blastema can be considered an indirect indicator of the dynamics of cell proliferation during regeneration.


Wormeter 1.0 allows you to characterize the morphogenesis of a blastema, which allows you to create an indirect , in vivo . method of assessing cell differentiation.
Wormeter 1.0 allows you to characterize the morphogenesis of a blastema, which allows you to create an indirect , in vivo . method of assessing cell differentiation. The program uses an approach to image analysis, based on the construction of a continuous morphological model of the silhouette of the planaria, and analysis of the disk cover of the silhouette - the union of all the disks of a given size inscribed in the figure.


Thus, an integral approach, called dynamic morphometry, has been developed for the quantitative recording of growth processes and morphogenesis.

 

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