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Abstracts

XVIII conference

Study of Antistress Oroperties of Plant Growth Regulator-Melafen in Terms of Model of Insufficient Watering

Zhigacheva I.V., Burlakova E.B., Misharina T.A., Terenina M.B., Krikunova N.N., Generozova I.P.¹, Shugaev A.G.¹

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334 Russia

¹Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, Russia

1 pp. (accepted)

Antistress properties of a plant growth regulator – melafen (melamine salt of bis(oxymethyl)phosphine acid) were studied in terms of a model of insufficient watering of pea sprouts. Insufficient watering leads to activation of lipid peroxidation (LPO) in mitochondria membranes of three-day sprouts. The intensity of fluorescence of LPO products increases almost threefold, which affects the physicochemical properties of the membranes and especially the fatty acids composition of the lipids. The relation of unsaturated fatty acids to saturated ones decreases 1.6fold for acids containing 18 carbon atoms and 3.3fold for acids containing 20 carbon atoms. A change in the fatty acids composition of mitochondria membranes is associated with changes in the energetics thereof: the maximum rates of oxidation of NAD-dependent substrates and the electron transfer rates at the end site of the respiratory chain decrease. The pretreatment of seeds with a 3х10-9М solution of melafen decreases the intensity of fluorescent of LPO products to control values. Moreover, a melafen treatment prevents changes in the fatty acids composition of mitochondrial membranes isolated from the sprouts. As a result, the maximum rates of oxidation of NAD-dependent substrates and the electron transfer rates at the end site of the respiratory chain are restored. The antistress properties of the agent manifest themselves also in experiments in whole plants. In particular, under conditions of insufficient watering, the seed germination of control plants decreases by 46%, whereas the germination of melafen-treated seeds almost does not vary. Moreover, melafen stimulates the growth of roots of sprouts under conditions of insufficient watering, which is an important adaptive effect.

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