Conference publications

Abstracts

XIX conference

Contents C20 unsaturated fatty acids in the mitochondrial membranes and stability of pea seedling to water temporary deficiency

Zhigacheva I.V., Burlakova E.B., Misharina T.A., Terenina M.B., Krikunova N.N., Generozova I.P.¹, Shugaev A.G.¹, Fattakhov S.G.²

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences,

¹Emanuel Institute of Biochemical Physics, Russian Academy of Sciences,ul. Kosygina, 4, Moscow, 119334 Russia

²Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya, 35, Moscow, 127276 Russia

³Arbuzov Institute of Organic and Physical Chemistry, Kazan’ Research Center, Russian Academy of Sciences, ul. Akademika Arbuzova, 8, Kazan’, 420083 Russia

1 pp. (accepted)

Temporary water shortage leads to changes in the fatty acid composition of mitochondrial membranes 6-day-old pea seedlings (Pisum sativum L). At the same time significant changes are observed in the content of fatty acids with 20 carbon atoms. Ratio of unsaturated fatty acids with 20 carbon atoms to the saturated reduced by more than 3 times. Changes in fatty acid composition of membranes appears to be related to the activation of lipid peroxidation (LPO): the fluorescence intensity of LPO products increased by 3.5 times.

This is accompanied by 30% reduction of the maximal rates of oxidation of NAD-dependent substrates and reduced efficiency of oxidative phosphorylation.

The treatment of seeds with 3х 10-12М melaphen prevents changes in the fatty acid composition of mitochondrial membranes caused by lack of moisture, and reduces to the control level of fluorescence intensity of LPO products. Melafen prevents due to water shortage changes in the efficiency of oxidative phosphorylation and restores the rate of oxidation of NAD-dependent substrates in the presence of ADP and / or FCCP. Between the index of unsaturation of C20 acids in the membranes of mitochondria and maximal rates of oxidation of NAD-dependent substrates is observed a strong correlation (Pearson's correlation coefficient is 0.9637).

It is suggested that the increase in content of unsaturated fatty acids, in particular the C20 acids in the membranes of plant tissues leads to increased resistance of plants to a lack of moisture.

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