Conference publications

Abstracts

XXIII conference

Influence of organosilicon compound on the functional state of mitochondria of plant and animal origin

Zhigacheva I.V., Burlakova E.B.

st. Kosigina,4, Moscow 119334, Russia

Tricyclic chelate silicon and germanium esters of triethanolamine with the General formula (RM(OCH2CH2)3N), where M= Si or Ge are quite promising compounds possessing wide spectrum of biological activity. Unlike silatranes, which are widely used in medicine and agriculture, germatranes, received considerably less attention. Although shown, germatranes have anti-stress properties, increasing the resistance of plants (wheat shoots) to temperature stress [Shigarova A. M. et al., 2013].

Since mitochondria play a major role in the organism’s response to the action of stressors studied the effect of different concentrations of 1- (germatran-1-yl) -1-oxyethylamine (germatrane) on the bioenergetic characteristics of mitochondria of animal and plant origin. Introduction 10-5- 10-11 M of the drug to the incubation medium of mitochondria resulted in increased efficiency of oxidative phosphorylation and to an increase of the maximum rates of NAD-dependent substrates and succinate oxidation by rat liver mitochondria. The drug at the same concentration stimulated the growth of the maximum speed of the oxidation of glutamate and malate by mitochondria 6 day etiolated pea seedlings (Pisum sativum L.) However, all test concentrations of the drug had no effect on the maximum rate of oxidation, or the rates of respiratory control in the oxidation of succinate by mitochondria of seedlings, which is probably indicative on the adaptive nature of the action of germatrane. Since mitochondria of germinating seeds are characterized by relatively low rates of oxidation of NAD-dependent substrates the result of maintenance high activity of NAD-dependent dehydrogenases is the support of the energy processes in cell that promotes the resistance of plant to varying environmental conditions [Generozova IP., Shugaev AG, 2012].

At the same concentrations (10-5- 10-11 M) germatrane had reduced the intensity of lipid peroxidation in the membranes of mitochondria of pea seedlings until control values in a model system of "aging" of the mitochondria and under water stress. Processing of seeds with 10-5 M solution germatrane has prevented slowdown of growth roots of seedlings under water stress

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